This study provides evidence for transovarial transmission of Rickettsia spp. when both pathogens were present in the nest. In fact, larvae had a significantly higher probability of being infected with Borrelia spp. phagocytophilum msp2/p44 gene copies detected on average, while Rickettsia-positive samples contained on average 5.4В Г—В 10 2 gltA gene copies. DNA in positive larvae was low, with 2.7В Г—В 10 0 Borrelia 5S-23S gene copies and 2.4В Г—В 10 1 A. Of the 1500 larvae, 137 were positive for Borrelia spp.

Overall, DNA from at least one pathogen could be detected in 90.0% (45/50, 95% CI: 78.2вЂ“96.7%) of the nests. Thirty-nine of 50 nests each (78.0%, 95% CI: 64.0вЂ“88.5%) were positive for Borrelia spp. phagocytophilum DNA using quantitative real-time PCR (qPCR). ricinus larvae from 50 collected вЂњnestsвЂќ (larvae adhering to the flag in a clumped manner) were individually examined for Borrelia, Rickettsia and A. In order to better estimate the potential infection risk by tick larvae for humans and animals, 1500 I. miyamotoi, whereas the efficiency of transovarial transfer under field conditions is largely unstudied. and Borrelia spp., especially regarding B. Under laboratory conditions, a transovarial transmission to the next tick generation is described for Rickettsia spp. Ixodes ricinus constitutes the main European vector tick for the Lyme borreliosis pathogen Borrelia burgdorferi ( sensu lato), the relapsing fever borrelia Borrelia miyamotoi, as well as Anaplasma phagocytophilum and several Rickettsia species.

Microsynth seqlab